mouse antitraf2 antibody  (Bio-Techne corporation)

Journal: Frontiers in endocrinology

Article Title: A novel link between chronic inflammation and humanin regulation in children.

doi: 10.3389/fendo.2023.1142310

Figure Lengend Snippet: FIGURE 4 Local effects of TNF on humanin, PCNA, SOX9 and TRAF2 expressions in growth plates tissue specimens (n=3) in patient 1 and patient 2 analyzed separately. Quantitative analysis of (A, E) humanin staining, (B, F) PCNA staining, (C, G) SOX9 staining, and (D, H) TRAF2 staining. Error bars indicate mean ± SE. Students t-test was used to analyze differences between groups.

Article Snippet: Next, slides were incubated with anti-humanin antibody (NB100-56877; Novus Biologicals, Littleton, Colorado, USA), anti-proliferating cell nuclear antigen (PCNA) antibody (ab-18197; Abcam, Cambridge, United Kingdom), anti-SOX9 antibody (ab-5355; Sigma-Aldrich, Burlington, MA, USA), and anti-TRAF2 antibody (NB100-56173SS; Novus Biologicals, Littleton, Colorado, USA) overnight at 4°C, 1:300 diluted for all antibodies.

Techniques: Staining

Journal: Frontiers in endocrinology

Article Title: A novel link between chronic inflammation and humanin regulation in children.

doi: 10.3389/fendo.2023.1142310

Figure Lengend Snippet: FIGURE 5 TNF suppressed SOX9, PCNA and TRAF2 expressions in human growth plate tissue specimens (n=6) obtained from 2 children or human chondrocytes. (A, B) Quantitative analysis of SOX9 staining (yellow arrows), calculated as number of positive cells per mm². (C) Relative expression of SOX9 assessed by qPCR in HCS-2/8 cells treated for 72 hours with TNF at 10, 30, 100, 300 ng/ml concentrations (n=3). (D, E) Quantitative analysis of PCNA staining (yellow arrows), calculated as number of positive cells per mm². (F) Relative expression of PCNA assessed by qPCR in HCS-2/8 cells treated for 72 hours with TNF at 10, 30, 100, 300 ng/ml concentrations (n=3). (G) Western blot analysis of SOX9 and PCNA expressions in HCS- 2/8 cells treated with TNF (100 ng/ml). (H, I) Quantification of SOX9 and PCNA expressions by three independent Western blot experiments. (J, K) Quantitative analysis of TRAF2 staining (yellow arrows), calculated as number of positive cells per mm². Error bars indicate mean ± SE. Students t- test was used to analyze differences between groups.

Article Snippet: Next, slides were incubated with anti-humanin antibody (NB100-56877; Novus Biologicals, Littleton, Colorado, USA), anti-proliferating cell nuclear antigen (PCNA) antibody (ab-18197; Abcam, Cambridge, United Kingdom), anti-SOX9 antibody (ab-5355; Sigma-Aldrich, Burlington, MA, USA), and anti-TRAF2 antibody (NB100-56173SS; Novus Biologicals, Littleton, Colorado, USA) overnight at 4°C, 1:300 diluted for all antibodies.

Techniques: Staining, Expressing, Western Blot

Journal: Frontiers in immunology

Article Title: The CD40 agonist HERA-CD40L results in enhanced activation of antigen presenting cells, promoting an anti-tumor effect alone and in combination with radiotherapy.

doi: 10.3389/fimmu.2023.1160116

Figure Lengend Snippet: FIGURE 3 Immunoprecipitation analysis of CD40 and associated proteins involved in signal transduction 15 mins post treatment. (A–C) Representative Immunoprecipitation and western blot analysis of immature dendritic cells differentiated from monocytes derived from PBMCs. Lysates were incubated with beads coated with the either CD40 (A), TRAF2 (B), or TRAF6 (C) antibodies, the indicated bound proteins to the respective target were detected via SDS-PAGE and then western blot analysis (n=3).

Article Snippet: IP of TRAF2 and TRAF6: Protein G Dynabeads were incubated with mouse-anti-human TRAF2 (R&D Systems Cat. No. MAB3277) or mouse-anti-human TRAF6 (R&D Systems Cat. No. MAB3284-100).

Techniques: Immunoprecipitation, Transduction, Western Blot, Derivative Assay, Incubation, SDS Page